基于多传感器融合技术的PCR仪温度校准系统

Temperature calibration system of PCR instrument based on multi-sensor fusion technology

提出了一种基于多传感器融合技术的PCR仪温度校准系统。系统对特征点的温度检测和处理后能给出PCR仪基座的温度均匀性和对称性,同时对采集到的多点温度数据运用多传感器数据融合技术进行处理,得到更接近真实基座温度的融合温度,从而可以得到仪器的温度示值误差,以实现对PCR仪的设定温度进行校准。系统选择LabVIEW作为开发软件,能自动生成检测报告。系统的温度校准不确定度优于0.03℃。实验表明,建立的温度校准系统可以用于目前大多数使用中的PCR仪温度的在线校准。

A temperature calibration system based on multi-sensor technology is introduced for the PCR instrument calibrations.The temperature uniformity and symmetry are calculated through acquiring and processing the temperature data at certain characteristic points on the temperature block of PCR.At the same time the temperature of the whole block is obtained using multi-sensor fusion technology,which is closer to the real temperature of the block and then the indication error of the instrument can be obtained.After evaluation of the measurement uncertainty,the temperature calibration of PCR instrument can be achieved.LabVIEW is chosen as the development software of the system and the calibrated results are reported automatically.The expanded uncertainty of the system in temperature calibration is around 0.03℃.The system can be used in on-line temperature calibration for most of PCR instruments.