大鼠同种异位心脏移植术后血清NO及心肌组织NOS活性的观察

Observation on serum nitric oxide and cardiac tissue nitric oxide synthase activity of heterotopic heart transplanted rats

目的 研究内源性一氧化氮（ＮＯ）与心脏移植急性排斥反应的关系。方法 本研究以大鼠同种异位心脏移植为研究对象，观察了术后３、５、７ 天的血清ＮＯ 水平以及心肌组织一氧化氮合成酶（ＮＯＳ）的活性。结果 在急性排斥反应发生的早期开始血清ＮＯ 水平就已显著升高（术后３、５、７ 天皆为Ｐ ＜ ０．０１）；心肌组织ＮＯＳ活性亦显著高于对照组（术后３ 天Ｐ ＜ ０．０５，术后５ 天Ｐ＜ ０．０１，术后７ 天Ｐ ＜ ０．０５）。结论 血清ＮＯ 水平的检测对于心脏移植急性排斥反应的发生可能具有早期的辅助诊断意义。

Objective To study the relationship between NO and acute rejection in heart transplantation.Methods We reproduced the heterotopic heart transplantation model of rats,the serum nitric oxide concentration and cardiac tissue nitric oxide synthase activity were measured 3 days,5 days and 7days after operation.Results The NO concentration in the serum was higher than that of the control group in the 3 days,5 days and 7days post operation;and NOS activity of cardiac tissue was also higher than that of the control groups in the 3days (P<0.05),5days(P<0.01) and 7days(P<0.05) post operation.Conclusions The NO concentration in serum may prove to be an effective criteria for monitoring acute rejection in heart transplantation. [