双酶酶解法提取姜油树脂工艺优化

Process Optimization for Double Enzymatic Extraction of Gingerol Oleoresin

研究采用纤维素酶和半纤维素酶共同酶解姜粉提取姜油树脂的最优提取工艺。以姜辣素的提取率为指标,通过单因素和正交试验,确定姜油树脂的最优提取工艺。结果表明:纤维素酶-半纤维素酶质量比5:3,混合酶用量2.8mg/100mL,酶解时间2.5h、酶解温度45℃。验证实验表明该方法所得姜辣素提取率3.711%,比乙醇溶剂浸提法的姜辣素提取率提高了30%。采用酶综合调控技术可以较高程度地提高姜辣素的提取率。

The present study was conducted to optimize the extraction of gingerol oleoresin from ginger powder by enzymatic hydrolysis with both cellulase and hemicellulase.By using one-factor-at-a-time and orthogonal array design methods,the optimal extraction conditions of gingerol oleoresin for achieving maximum extraction efficiency were determined as follows： ellulase-to-hemicellulase ratio of 5：3（m/m）,total enzyme dose of 2.8 mg/100 mL,hydrolysis time of 2.5 h and hydrolysis temperature of 45 ℃.Under the optimum conditions,the extraction efficiency of gingerol oleoresin was 3.711%,30% higher than the yield of ethanol extraction.