大孔吸附树脂分离纯化洋葱皮黄酮的研究

Separation and Purification of Flavonoids from Onion Peel by Macroporous Resin Adsorption

以黄酮含量为指标,比较6种大孔吸附树脂对洋葱皮黄酮的吸附和解吸效果。通过静态吸附与解吸实验,筛选出效果较好的X-5树脂进行动态实验研究。结果表明:X-5树脂纯化洋葱皮黄酮的工艺条件为吸附流速2mL/min、上样溶液pH5.0、上样液质量浓度0.5mg/mL、50mL 80%乙醇作为洗脱液,洗脱流速lmL/min。经X-5树脂纯化后,洋葱皮黄酮纯度从7.95%提高到80.78%。三次重结晶后其纯度可达94.5%。该方法简单可行,纯化效果好,适合于工业化生产。

The characteristics of adsorption and desorption of onion peel flavonoids on six kinds of macroporous resins were compared.Static adsorption and desorption experiments indicated that X-5 resin was the best resin for the separation and purification of flavonoids from onion peel.The optimal process parameters for purifying onion peel flavonoids with X-5 resin were sample loading flow rate of 0.5 mg/mL,sample pH 5.0 and 50 mL of 80% ethanol of as eluent at a flow rate of 1.0 mL/min.After the purification,the purity of flavonoids from onion peel was increased from 7.95% to 80.78% and could reach up to 94.5% after further triple recrystallization.The developed method is simple and feasible for industrialization.