甲型H1N1(2009)流感流行后流感样病例呼吸道病原检测分析

Respiratory pathogens Detection of influenza-like cases after influenza pandemic of Influenza A H1N1 in 2009

目的:了解甲型H1N1(2009)流感流行后的流感样病例(ILI)中,除流感病毒外的相关病原分布,为ILI患者临床治疗、流行病学调查提供参考。方法:收集流感监测哨点医院393份ILI咽拭子标本提取核酸,应用实时荧光PCR方法进行20种相关病原比对检测,分析各病原的阳性率与构成比。结果:检测出阳性咽拭子标本51例,分属13种共计64株病原体,其中阳性率较高的为肺炎链球菌、流感嗜血杆菌、冠状病毒。另有13例为两种病原体混合感染,占阳性标本数的25.49%。结论:ILI的病原构成比较复杂,且存在混合感染,临床治疗与流行病学调查均应引起重视。

Objective：To detect the pathogens expect influenza virus in the specimens from the tracheal aspirate of the patients with influenza-like illness（IL I） after the influenza A H1N1 pandemic in 2009,and to characterize the etiology spectrum which could be a useful reference for clinical therapy and epidemic surveillance.Methods： RNA/DNAs were extracted from 393 throat swab specimens of the patients with ILI.20 species of pathogens were comparison detected by real-time PCR,and the positive rate of each pathogen and the ratio of different pathogens were analysed.Results： 64 pathogens in 13 categories were detected in the 51 specimens.The positive rate of Streptococcus pneumoniae,Haemophilus influenzae and coronavirus were obviously high.There were 13 cases with mixed infections of two pathogens and which accounts 25.49 percent in positive patients.Conclusions： The results suggested that the pathogens of the patients with IL I were multiplex.The opportunistic infections and mixed infections were commonly The rapid and sensitive detection methods are suggested to be carried out to assist clinic diagnosis.And the combined medication should be used in the treatment of patients.