口腔颌面部恶性肿瘤尿激酶及其受体的基因表达和临床意义

Gene expression and significance of urokinase type plasminogen activator and its receptor in oral and maxillofacial tumor tissue

目的：探讨ｕＰＡ及ｕＰＡＲ与口腔颌面部恶性肿瘤浸润、转移的关系。方法：应用ｃＤＮＡ－ｍ ＲＮＡ斑点杂交技术定量检测３０ 例口腔颌面部恶性肿瘤ｕＰＡ和ｕＰＡＲｍ ＲＮＡ表达水平。结果：癌组织ｕＰＡ和ｕＰＡＲｍ ＲＮＡ含量高于相应正常组织（Ｐ＜ ０．０１），伴有淋巴结转移癌组织ｕＰＡ 和ｕＰＡＲ高于无淋巴结转移者（Ｐ＜ ０．０１，Ｐ＜ ０．０５），ｕＰＡ、ｕＰＡＲ表达水平与淋巴结转移关系密切（ｒ＝ ０．４１ Ｐ＜ ０．０１，ｒ＝ ０．３１ Ｐ＜ ０．０５），与Ｔ分期、分化程度及肿瘤类型无明显关系。结论：ｕＰＡ 和ｕＰＡＲ是促进口腔颌面部恶性肿瘤转移的重要因素，其表达水平可作为肿瘤转移的参考指标。

Objective:To investigate the relationship between gene expression levels of urokinase type plasminogen activator (uPA) & its receptor (uPAR) and biological behavior of oral and maxillofacial malignancies.Methods:The mRNA contents of uPA and uPAR in samples were quantitatively detected by cDNA mRNA dot blot hybridization. Results:The malignent tumors had greatly higher mRNA contents of uPA and uPAR than those in their relevant surrounding tissues ( P <0 01). These mRNA contents in cancer tissues with lymphatic metastases were higher than those without lymphatic metastases ( P <0 01 and P <0 05); lymphatic metastases were mainly affected by these genes expression levels ( r =0 41 P <0 01, r =0 31 P <0 05). T classification, differential degradation or tumor kind wasn′t correlated with uPA or uPAR mRNA content. Conclusion:The overexpression of uPA and uPAR gene is an important factor in the metastases of promoting oral and maxillofacial malignent tumors. It may be used as a symbol of tumor metastasis to detect these mRNA contents.