持续埋植褪黑激素对SOX21基因表达影响研究

Study on Effect of Constant-release Melatonin on SOX21 Gene

试验通过对内蒙古绒山羊采用持续埋植两年褪黑激素和提取皮肤总RNA后,进行Real-time PCR扩增,并研究了褪黑激素(melatonin,MT)对绒相关基因SOX21在皮肤中表达的影响。以GAPDH基因为看家基因对荧光定量获得的相对定量的数据进行校正,数据经2-△△Ct法分析,结果发现:①SOX21基因在不同个体中的表达趋势是相对稳定的,不受个体差异的影响。②褪黑激素对一年中SOX21基因在内蒙古白绒山羊皮肤中表达的影响除3、4、5月份外其他月份均有显著的影响。③持续埋植两年稳定了褪黑激素对SOX21基因在绒山羊皮肤中表达的影响,降低了绒山羊对褪黑激素的应激反应。④SOX21基因在毛囊休止期表达明显下调,可能与脱绒相关。

The experiment by the way of constant-release melatonin two years and abstract skin total RNA Real-time PCR to Inner Mongolia Cashmere goats,for depth study melatonin effects on SOX21 gene.Used GAPDH gene as housekeeping gene to correct the quantitative data relative which obtain by Real-time PCR,the data by 2-△△Ct analysis found that： ① SOX21 gene in different individuals expressions in the trend was relative stability and variability impact of hormones.② There were significant effect in addition to March,April,May in the SOX21 gene expression was no trend significant change,the other months had changed significantly.③ While constant-release melatonin on the SOX21 gene expression,reducing the goat of the stress response to melatonin.④The SOX21 gene express was down regulation in the hair follicle telogen.