Skp2、Caspase-3在膀胱尿路上皮细胞癌组织中的表达

Expression of Skp2 and Caspase-3 in Human Bladder Transitional Cell Carcinoma

目的检测Skp2、Caspase-3在膀胱尿路上皮细胞癌(简称膀胱癌)组织中的表达,探讨其临床意义及相关性。方法采用免疫组化方法检测Skp2、Caspase-3蛋白在82例膀胱癌组织和16例正常膀胱黏膜组织中的表达,并收集临床资料,分析检测结果与临床病理的联系及二者的相关性。结果 Skp2在膀胱癌组织中的阳性率(43.9%)高于正常膀胱黏膜组织(12.5%)(P<0.05);Caspase-3在膀胱癌组织中的阳性率(39%)低于正常膀胱黏膜组织(81.3%)(P<0.05)。膀胱癌细胞的恶性程度越高,Skp2蛋白表达越高,Caspase-3则无明显差异;与非肌层浸润性膀胱癌相比,肌层浸润性膀胱癌组织中Skp2蛋白的表达较高(P<0.05),而Caspase-3的表达则较低(P<0.05)。膀胱癌组织中Skp2蛋白的表达与Caspase-3成负相关关系。结论 Skp2蛋白的表达与膀胱癌的分化、临床分期密切相关,与Caspase-3的表达成负相关;联合检测Skp2、Caspase-3有助于判定膀胱癌的侵袭能力。

Objective To detect the expression of Skp2 and Caspase-3 in bladder transitional cell carcinoma（BTCC） and investigate their clinical significance and dependablity.Methods Eghty-two cases of bladder transitional cell carcinoma tissue and 16 cases of normal bladder epithelium were evaluated for Skp2 and Caspase-3 protein expression by immunohistochemical method,analyze the correlation between the outcome of detection and clinicopathologic features.Results The positive expression level of Skp2 in BTCC was higher than that in normal bladder epithelium.While Caspase-3 was lower.In bladder cancer,the positive expression level of Skp2 was positively correlated with the degree of malignant and clinical stage;the positive expression level of Caspase-3 was negatively correlated with clinical stage,but there was no correlation with the degree of malignant;Skp2 level negatively correlates with Caspase-3 level.Conclusion Increased expression of Skp2 is markedly correlates with clinical classification and differentiation of BTCC,and negatively correlates with the expression of Caspase-3.Combined detection of Skp2 and Caspase-3 may be helpful to evaluate aggressive behavior of BTCC.