输血传播病毒在原发性肝癌患者中的感染状况研究

Prevalence of transfusion transmitted virus infection in patients with primary liver cancer

目的 了解新型肝炎病毒输血传播病毒 (transfusiontransmittedvirus,TTV)在原发性肝癌 (PLC)患者中的感染状况。方法 在TTV长的ORF保守区设计引物 ,建立巢式聚合酶链反应 (PCR)技术 ,检测了 15 1例原发性肝癌患者血清中TTVDNA。结果 15 1例原发性肝癌患者中有 2 8例TTVDNA阳性 ,阳性率为 18 5 % ;而同时检测 12 5例正常人血清只有 3例TTV阳性 ,阳性率为 2 4 %。结论 TTV在原发性肝癌患者中存在 。

Objective To investigate the prevalence of transfusion transmitted virus (TTV) in patients with primary liver cancer (PLC).Methods A nested polymerase chain reaction (PCR) assay with primers from ORF1 of TTV genome was established to detect TTV DNA in sera of 151 PLC patients and 125 controls.Results 28 of 151 (18 5%) PLC patients and 3 of 125 (2 4%) controls were positive for TTV DNA ( P <0 005).Conclusion Prevalence of TTV DNA in the patients with PLC is significantly higher than that in controls. The nested PCR assay of TTV DNA is a useful technique for studying relationship between TTV infection and PLC.