糖尿病大鼠红细胞对脑组织损伤的影响

Changes of Erythrocytes in Diabetic Rats and Their Effects on Damage of Normal Brain

研究糖尿病时红细胞的变化对脑组织损伤的影响。方法：用链脲佐菌素（ＳＴＺ，５０ｍｇ／ｋｇ）诱发大鼠糖尿病，分离红细胞，输注给正常大鼠（８×１０９个红细胞／只），测定脑组织ＭＤＡ和亚硝酸盐含量，同时将分离红细胞在体外用异硫氰酸荧光黄（ＦＩＴＣ）标记，荧光显微镜观察输入的红细胞在脑微血管中的流动。结果：ＳＴＺ大鼠血糖持续高于正常，病程达一年的大鼠糖基化血红蛋白（％）明显高于正常大鼠（Ｐ<０．０１）。将糖尿病１年大鼠红细胞输给正常大鼠，４ｈ后，糖尿病红细胞组大鼠脑组织ＭＤＡ和亚硝酸盐含量明显高于对照红细胞组（ＭＤＡＰ＝０．０３２；亚硝酸盐Ｐ＝０．０２７）。软脑膜微循环的活体观察发现有ＦＩＴＣ标记的糖尿病红细胞粘附于微血管壁。结论：糖尿病的红细胞可以直接引起正常脑组织自由基代谢产物的变化，其作用可能与红细胞对内皮细胞的粘附有关。

To examine the changes of erythrocyte in diabetic rats and myestigate further the effects of diabetic erythrocytes on brain damage. Methods: Diabetes in rats were induced by STZ, diabetic erythrocytes were isolated andtransfused into blood of normal rats, the contents of MDA and nitrate in transfused tats brain tissue were measured. Flowing status of tansfused RBC withincerebral microvessels were observed under fluorescent microscope. Results: Erythrocyes from rats with diabetes for 1 year were isolated and transfused into mormal rats, the content of MDA and nitrate of brain tissue were measured 4 hoursafter RBC transfusion. The level of MDA and nitrate in group of rats receiveddiabetic erythrocytes were significant higher than that group of rats received controlled ROC(MDA P=0. 032, nitrates P=0. 027 ). The FITC-labelled diabeticerythrocytes were revealed in vivo adhering to endothelial cells of microvesselsin pial microcirculativn. Conclusions: Erythrocytes from diabetic rats can induce the increase of MDA and nitrate level in brain tissue of normal rats, whichmay contribute to brain damage in diabetes, and their effects could be related toadhesion of erythrocyte to endothelial cell.