提高染色体分散度的方法研究

The methods to improve Chromosome dispersion

染色体的良好扩散是成功进行复杂分子细胞遗传学研究的关键。为更深入地了解中期染色体扩散的动力学特征,本实验探讨了细胞悬液的滴片高度,滴片的湿度和角度,干燥的时间,固定液的比例,玻片的温度,相对的湿度这些可变因素对中期染色体扩散的影响。

Good chromosome spread of a successful genetic studies of complex molecular and cellular key.To better understand the dynamic characteristics of metaphase chromosome spread,investigated the effects of the cell suspension drops pieces height and angle of droplets film humidity,drying time,the ratio of fixative,the slide temperature and relative humidity in the Variables on the impact of the spread of metaphase chromosomes.