L-天冬酰胺酶Ⅱ基因工程菌的培养

Cultivation of Engineering Bacteria Producing L-Asparaginase II

为提高L 天冬酰胺酶Ⅱ基因工程菌EscherichiacolipKA/CPU2 10 0 0 9的产酶量 ,采用正交试验法确定了较佳培养条件 ,并优化了发酵工艺。工程菌摇瓶产酶稳定在 190U/ml。 2 5L发酵罐产酶亦高达 180U/ml,发酵周期大大缩短 ,仅 9～ 10h。产酶量比原工艺提高 1倍以上 ,具有较好的工业生产前景。

In order to improve the enzyme production of engineering bacteria Escherichia coli pKA/CPU 210009, a suitable media has been found by orthogonal design and some fermentation conditions were optimized Enzyme production of engineering bacteria maintained 190U/ml in flasks and 180U/ml in a fermentor Fermentation period shortened greatly in a fermentor Enzyme production from the suitable media increased 2 fold than in original fermentation conditions