安多霖对微波辐射致大鼠胸腺和脾脏组织学及超微结构损伤的预防作用研究

Precautionary effect of AduoLa Fuzhenglin on ultrastructural and histological injury to rats′ thymus and spleen induced by microwave exposure

目的观察安多霖对微波辐射致大鼠胸腺和脾脏组织学及超微结构损伤的预防作用。方法 Wistar雄性大鼠280只,随机分为短期(14 d)及长期(28 d)安多霖预防组,每组140只。每组又随机分为空白对照组、辐射组及0.75、1.5、3 g/(kg.d)安多霖预防组。辐射组及预防组动物连续灌胃给药14 d(每天1次)后进行30 mW/cm2微波辐射15 min,于停药(辐射)后6 h、7 d及14 d将动物分批活杀,采用光镜及电镜观察胸腺和脾脏的组织学及超微结构改变。结果短期安多霖预防组:停药后6 h及7 d,辐射组及0.75 g/(kg.d)安多霖预防组大鼠脾脏组织学及超微结构明显损伤,胸腺无明显改变;1.5及3 g/(kg.d)安多霖预防组:辐射后6 h及7 d,脾脏改变较辐射组明显减轻,停药后14 d各组病变基本恢复;长期预防组改变与短期预防组规律相同。结论预防性给予1.5 g/(kg.d)安多霖可对30 mW/cm2微波辐射引起的大鼠免疫器官结构损伤有明显的改善作用;给药28 d与给药14 d效果无明显差异。

Objective To investigate the precautionary effect of AduoLa Fuzhenglin（ADL） on the histological and ultrastructural injury to rats′ thymus and spleen induced by microwave exposure.Methods Two hundred and eighty male Wistar rats were randomized into short term（14 d） and long term（28 d） ADL preventin groups,with 140 rats in each.Each group was randomized into five subgroups,three of which had been treated with 0.75,1.5 and 3 g/（kg·d） ADL before they were exposed to 30 mW/cm2 microwave radiation for 15 min.The subgroups without ADL treatment or radiation were used as control,and the one with radiation alone as radiation subgroup.The changes in histology and ultrastructure in rats′ thymus and spleen were observed by light and transmission electron microscopy at 6 h,7 d and 14 d after microwave exposure.Results In short-term group,obvious histological and ultrastructural damage to rats′ spleen of radiation subgroup and 1.5 g/（kg·d） ADL subgroup were observed at 6 h and 7 d after administration of ADL was stopped.The pathological changes in 1.5 g/（kg·d） and 3 g/（kg·d） ADL subgroups improved significantly at 6 h and 7d after ADL was suspended and the injuries recovered nearly at 14 d.Long-term group was similar to short-term one.Conclusion ADL has proved to protect against the structural injury to the spleen caused by 30 mW/cm2 microwave exposure.1.5 g/（kg·d） is an effective and economical dosage.Giving ADL for 14 d has the same effect as giving ADL for 28 d.