含笑花精油的抑菌活性及其化学成分分析

Study on antimicrobial activity and chemical constituents of essential oil from the flowers of Michelia figo Spreng

运用水蒸气蒸馏法提取含笑花精油,分别采用平板菌落法和滤纸片法检测精油对细菌和真菌的抑菌活性,并运用GC/MS法分析精油的化学成分。结果表明:含笑花精油对枯草芽孢杆菌、大肠杆菌和总状毛霉抑制效果较佳,抑菌率分别达70.36%、60.24%和65.52%;对金黄色葡萄球菌、黄曲霉具有一定的抑制作用,对米根霉无抑制效果;从含笑花精油中分离鉴定出30种化合物,其中脂肪族化合物14种、萜烯类化合物16种。

Essential oil from the flowers of Michelia figo was extracted by steam distillation and the antimicrobial activities of the essential oil on bacteria and fungi were tested by plate counts and filter paper methods. Then the chemical constituents of the essential oil were analyzed by GC/MS. The results showed that： the antimicrobial effect of the essential oil from the flowers of M. figo on Bacillus sabtills, Escherichia coli and Mucor racemosus were good and their inhibitory rates were up to 70.36%, 60.24% and 65.52%. There were certain antimicrobial effect of the essential oil on Staphylococcus aureus and Aspergillus flavus, but no antimicrobial effect on Rhizopus oryzae. Thirty compounds were separated and identified from the essential oil including 14 aliphatic compounds and 16 terpenoid compounds.