-80℃简易冻存外周造血干细胞临床应用观察

Simple saves frozen of peripheral blood stem cells at -80℃

目的:探索一种简便易行、安全有效、成本低的干细胞的冻存方法。方法:6份外周造血干细胞以1640培养液和二甲基亚砜、10%白蛋白作保护剂在-80℃条件下直接冻存,检测冻存前后外周血干细胞的活性、单个核细胞(MNC)数和CD34+细胞数及回收率。结果:冻存前后外周血干细胞活性、MNC和CD34+细胞的数量及回收率满足移植要求,输注后造血均重建。结论:1640培养液和二甲基亚砜、10%白蛋白作保护剂在-80℃条件下直接冻存干细胞是一种简便、可靠、安全、成本低的外周血干细胞冻存方法。

Objective To search for a simple,safe and effective,low-cost method of frozen stem cells.Method Six peripheral blood stem cells（BPSC） directly at-80℃ by using 1640 and DMSO,10% albumin as protection,before and after frozen,examinate of the PBSC activeness,the number of mononuclear cells（MNC） and CD34＋ cells and recovery rate.Results The BPSC activeness,the number of mononuclear cells（MNC） and CD34＋ cells and recovery rate satisfied the transplant request,hemopoiesis could be reconstructed after infusion all.Conclusion Freezing stem cells directly at-80℃ by using 1640 and DMSO,10% albumin as protective agents is a convenient,reliable,safe and low cost procedure to store PBSC.