15例急性淋巴细胞性白血病微小残留病追踪检测结果分析

Analysis on follow-up assay of minimal residual disease in acute lymphoblastic leukemia (ALL) in children

为探讨残留白血病细胞的存在与白血病患儿长期生存之间的关系,我们用非同位素的PCR-RNA转录本分子杂交法,选用T细胞抗原受体γ链(TCR_γ)重排基因为标志,检测了20例急性淋巴细胞白血病(ALL)患儿,并对其中具有TCR_γ重排基因的15例患儿进行了追踪检测,结果显示:化疗后早期(<5月)大多数患儿都能检测到残留白血病细胞,长期缓解者微小残留病检测为阴性。结果表明,当临床达到完全缓解时,对每1例患者的残留白血病细胞进行监测是必要的。

It is generally accepted that minimal residual disease is one of the primary reasons for the recurrence of acute leukemias in children. In order to explore the relationship between the existence of residual leukemic cells and long-term survival of children with ALL, using T-cell antigen receptor γ-chain (TCγ) gene rearrangement as the marker, the non-radiating PCR analysis of RNA-transcripting molecule hybridixation has been prospectively carried out in 20 children with ALL, of which 15 cases having TCRy gene rearrangement were followed up in the disease course all the results obtained showed that residual leukemic cells were found during early period after the start of chemotherapy (<5 months) in most of the patients with ALL) meanwhile, minimal residual disease could be interpreted in affected children with long-term remission. Through these results, the authors claim that it is very neccessary for each patient with clinical complete remission to perform the assessment of residual leukemic cells.