巨细胞病毒UL44、UL32特异性免疫球蛋白M的检测在婴儿巨细胞病毒性肝炎中的意义

Determination of specific cytomegalovirus UL44-IgM and UL32-IgM and its clinical significance in infants with CMV hepatitis

为探讨巨细胞病毒(CMV)UL44(P52)、UL32(P150)特异性免疫球蛋白M(抗P52-IgM、抗P150-IgM)与CMV肝炎的临床关系。运用免疫印迹技术(IB)对26例CMV肝炎患儿外周血特异性抗P52-IgM、抗P150-IgM进行检测。结果:26例患儿中抗P52-IgM阳性率为100％,抗P150-IgM阳性率为61.5％;先天感染抗P150-IgM阳性率明显高于围生期感染(P<0.01);围生期抗P150-IgM阳性患儿其白细胞计数、直接胆红素值、γ-谷氨酰转肽酶值、锁骨中线肋下肝肿大值均高于抗P150-IgM阴性患儿(P分别<0.01、0.01、0.01、0.05)。提示抗P52-IgM和抗P150-IgM均阳性与疾病严重性有一定关系,抗P52-IgM阳性在确诊CMV肝炎中有很高的敏感性。

To explore the clinical relationship between specific cytomegalovirus UL44(p52)-IgM and UL32(pl50)-IgM (anti-p52-IgM and anti-pl50-IgM) and cytomegaloviral hepatitis. Serous specific anti-p52-IgM and anti-p150-IgM were determined in 26 infants with cytomegaloviral hepatitis by using Immunoblot (IB). The results showed that the positive rates of anti-p52-IgM and anti-pl50-IgM in these infants were 100% and 61.5%, respectively. The positive rate of anti-p52-IgM in infants with congenital infection was more higher that with perinatal infection (P<0. 01). The leucocyte count, direct biliru-bin level, 7-glutamyltranspeptidease level and hepatomegaly were all higher in the infants with positive anti-p52-IgM than that in the negative one (P<0. 05 or 0. 01). It is suggested that positive anti-p52-IgM and anti-pl50-IgM play an important role in the severity of disease, in particular anti-p52-IgM is more sensitive in the diagnosis of cytomegaloviral hepatitis.