长果种黄麻SRAP标记遗传连锁图谱的构建及3个质量性状基因定位

Construction of a Molecular Linkage Map Using SRAP and Mapping of Three Qualitative Traits in Corchorus olitorius L.

【目的】构建黄麻遗传连锁图谱,定位质量性状基因,为今后有关黄麻基因组结构、重要农艺性状QTL定位、分子标记辅助育种和基因克隆等研究工作奠定基础。【方法】以甜麻(黄麻野生种)和宽叶长果(黄麻栽培品种)为杂交亲本,构建了187个F2单株作为作图群体,利用513对SRAP引物进行遗传图谱构建,并对3个质量性状基因(托叶色、叶柄色、叶缘色)进行了定位。【结果】122个SRAP多态性标记位点和这3个形态学标记被定位在该图谱上,初步构建的长果种黄麻遗传连锁图谱全长2 231.9 cM,包含10个连锁群,每个连锁群有2—38个标记位点,2个标记间平均间距为17.86 cM。【结论】该图谱上的标记位点均匀分布在10个连锁群上,没有出现标记位点聚集的现象,表明SRAP标记十分适合黄麻遗传图谱的构建。

【Objective】Jute（Corchorus olitorius L.） is one of the most economically important crops for fiber production.The present study was aimed to construct a molecular linkage map of jute and locate important qualitative traits,which will provide essential tools for investigating genomic structure and evolution,facilitating QTL mapping,marker-assisted selection and map-based cloning of important gene.【Method】In this study,a population of F2 generation including 187 individual plants was derived from the cross between Corchorus aestuans L.（wild species） and wild leaf jute（cultivated species）.A total of 513 sequence-related amplified polymorphisms（SRAP） primer pairs were used in constructing the molecular genetic linkage map and mapping 3 qualitative traits including color of stipule,color of stipe,and color of leaf margin.【Result】This genetic linkage map containing 10 linkage groups（LGs） was constructed using 122 SRAP loci and these 3 morphological markers,the map covered 2 231.9 cM with the average marker interval of 17.86 cM,every linkage group included 2-38 markers.【Conclusion】All the markers are distributed evenly among the linkage groups without clustering of loci.SRAP markers may be a better type of markers in map construction.