HBV S基因亚型与前C基因1896位点变异关系的初步研究

Study on Correlation of HBV S-gene Subtype with Precore Gene 1896 Point Mutation

目的:研究HBV S基因亚型与前C基因1896位点变异的关系。方法:对104例病人血清用PCR技术特异性扩增前C基因1896位点突变和用PCR-RFLP技术对HBV S基因分型。结果:①1896位点变异检出率为63.46％;②武汉地区HBVS基因亚型Ⅰ感染率为68.27％,亚型Ⅱ为25.00％,亚型Ⅲ为4.81％,亚型Ⅰ+Ⅱ复合感染为1.92％;③在亚型Ⅱ中,单纯野生株感染为80.77％,单纯变异株为7.7％,野生株变异株同时感染为11.54％,而占感染多数的亚型Ⅰ中,野生株和变异株的感染无差异。结论:1896位点是高频率变异位点,其变异不是随机的,而是与HBV不同的S基因亚型有关,基因亚型Ⅱ不易发生1896位点变异,受感染的机体内环境只是起促进变异的作用。

Aim: To study the relevance of HBV S-gene subtype with precore gene 1896 point mutation. Methods: Examining precore gene 1896 point mutation used PCR and typing HBV S-gene subtype used PCR-RFLP. Results: ① Ratio of precore gene 1896 point mutation was 63. 46%; ②Subtype distribution: subtype Ⅰ ,was 68. 27%; subtype Ⅱ 25. 00% ; subtype Ⅲ4. 81 %; co-infection of subtypeⅠ with subtypeⅡ was 1. 92% ; ③In subtypeⅡ, ratio of wild type infection was 80. 77% , ratio of mutant type was 7. 70%. co-infection of wild type and mutant type was 11. 54%, but in subtype Ⅰ,ratio of wild type infection was approximate with mutant type infection. Conclusion: Precore gene 1896 point mutation was high frequent and un-random, it seemed associated with the type of S-gene subtype, precore gene 1896 point mutation occurred hardly in HBV S-gene subtype Ⅱ .