蜜环菌水溶性多糖的分离鉴定与发酵条件优化

Isolation of water-soluble polysaccharide and optimization of fermentation conditions from Armillaria mella

目的:筛选出产蜜环菌多糖和菌丝体生长的最适碳氮源,对蜜环菌水溶性多糖进行结构和组成分析。方法:改变蜜环菌发酵的不同种类碳氮源,测定多糖含量和菌体干重,经热水浸提、除蛋白等步骤分离纯化到水溶性多糖,利用紫外、红外光谱、气相衍生化法分析其基本结构信息。结果:红薯粉和豆粕的组合最适合产多糖,玉米浆和玉米粉的组合菌丝体得率最高;多糖结构由吡喃葡萄糖组成。结论:在富含天然类成分的培养基中,蜜环菌菌丝体生长和产多糖能力较高;提取到的水溶性多糖,成分单一;糖醇乙酸酯气相衍生化法可简便、准确地测出蜜环菌多糖中所含的各种单糖。

Objective：In order to find out the optimal carbon and nitrogen sources of the polysaccharide production and strain growth from Armillaria mella.We isolated and purified the water-soluble polysaccharide from Armillaria mellea,and further analyzed its structure and composition.Methods：With different types of carbon and nitrogen sources were changed,we measured the polysaccharide production and dry cell weight in Armillaria mellea＇s fermentation.The methods of hot water extraction and proteins removed were applied to get the pure polysaccharide.We idendified the basic structure of monosaccharides by UV-spectroscopy,infrared spectroscopy and gas chromatography（GC） in the condition of derivatization.Results：The polysaccharide production in the combination of sweet potato power and soybean power was the best.Corn syrup and corn meal was a combination of the highest yield of mycelium.The structure of polysaccharide is composed by a single glucopyranose.Conclusion：Natural class of medium are more suitable for strain growth and polysaccharide production.We exacted the water-soluble polysaccharide in a simple way and its component is single.The GC derivative method is simple and accurate,and it can be used for analysis of various monosaccharide from polysaccharides.