高脂饮食诱发大鼠胰岛素抵抗后肿瘤坏死因子-α的改变

Changes of tumor necrosis factor-α concentrations in high fat fed induced rat insulin resistance model

目的 了解高脂饮食所致的大鼠胰岛素抵抗（ＩＲ）模型血及组织中肿瘤坏死因子－α（ＴＮＦ－α）含量的变化。方法 用脂肪占摄入热卡６１％ 的饮食饲养大鼠７周，以葡萄糖－胰岛素耐量试验（Ｇ－ＩｎｓＴＴ）筛选出ＩＲ大鼠，以放射免疫法分别检测其血清、肝脏、红色股四头肌、脂肪组织匀浆中ＴＮＦ－α的含量。结果 高脂饮食致ＩＲ大鼠血及组织中ＴＮＦ－α含量均明显升高，且其升高程度与高胰岛素血症呈正相关，与Ｇ－ＩｎｓＴＴ中的Ｋ值呈负相关。结论 高脂饮食的ＩＲ大鼠中存在ＴＮＦ－α合成和／或分泌的升高。

Objective To study the changes of serum and tissue TNF α concentrations in high fat fed induced rat insulin resistance model.Methods S D rats were fed equicalorically with a high fat diet (fat 61% of total kcal) for 7 weeks, then insulin sensitivity was measured with glucose insulin tolerance test. In rats with insulin resistance, the TNF α concentrations in serum, liver, red quadriceps and adipose tissues were estimated by radioimmunoassay.Results The serum and tissue TNF α concentrations were all significantly increased in high fat fed rats with insulin resistance, and it is positively correlated with serum insulin level and negatively correlated with K values obtained from glucose insulin tolerance test. Conclusion The synthesis and/or secretion of TNF α is increased in high fat fed rats, and is closely correlated with the occurrence of insulin resistance.