HPLC法测定蒸汽爆破玉米秸秆水解液中的糖含量

Pretreatment and determination of sugars in the hydrolysate after stream explosion of corn stover

热水抽提蒸汽爆破处理后的玉米秸秆,利用高效液相色谱仪,AminexHPX-87P离子色谱柱为分析柱,示差折光检测器分离分析所得水解液中的主要糖分：木糖、葡萄糖、纤维二糖、半乳糖、阿拉伯糖、甘露糖。通过比选各种样品前处理方法及色谱条件优化,选定了添加适量三氯乙酸做样品前处理,检测采用85℃柱温和超纯水作为流动相,水解液样品中各组分的线性相关系数均在0.9990-0.9999之间,回收率为86.3%-109.4%,精密度为1.14%-4.64%。在样品中糖损失最小的情况下,有效去除了色素、脂类、蛋白等影响测定并损害色谱柱的污染物,在最佳色谱条件下,蒸汽爆破水解液中的主要糖分能够同时被测定。

The sugar group in the hydrolysate of steam explosion were analysed by HPLC with a refractive index（RI） detector and a Bio-Rad HPX-87H column.The mobile phase was 5 mmol/L sulfuric acid with the flow rate of 0.6 mL/min and the column temperature was 85 ℃.Pretreat methords were also been compared and chosen.After treated by active carbon or trichloroacetic acid,under the optimal chromatogram conditions,sugars were completely separated and simultaneously determined.All the correlation coefficients were between 0.9990 and 0.9999 with the recovery ranged from 86.3%~109.4%.And the relative standard deviation was in the range of 1.14%~4.64%.