摘要
探讨食管癌患者癌组织和癌旁间质中Lc、Tc的密度与组织学分类、生物学行为及与预后的关系。方法采用S-100、CD3抗体和免疫组织化学ABC方法,对249例原发食管癌分别标记,观察癌巢内和癌旁间质中Lc、Tc的形态、分布特点和密度。结果Tc阳性细胞阳性定位在细胞核和细胞质,不均质棕褐色颗粒。Tc阳性细胞阳性定位在胞膜呈棕黄色。癌巢内Lc数量与肿瘤分化程度、浸润深度、淋巴结转移至正相关,与患者生存期呈负相关。而Tc数量和癌旁间质Lc数量则相反。Lc、Tc分布数量与组织学分类无关。结论Lc、Tc在癌巢内和癌旁间质分布密度可作为判定食管癌预后的免疫学指标。
Objective To investigate the relationship between the density of langerhan cell(Lc) and Tcell (Tc) and the histological classification, biological behavior and prognosis of esophageal carcinoma.Methods The immunohistochemical (ABC) method with S-100 and CD3 staining was used in samples ofprimary esophageal cancer in 249 cases for quantitative analysis. All cases were followed up for more than7 years. We observed the morphology,number and distribution of langerhan cell (Lc) and T cell (Tc) indifferent portionof the tumor mass. Results Lc positive expressed in the nucleus and cytoplasma. To positiveexpressed in cytomembrane. The number of Lc in cancer nest had positive correlation with malignantdegree of tumor, infiltration depth and metastasis of lymph node, and had negative correlation with thesurvival period. On the contrary, the number of To and Lc in peritumor interstitial tissue had negativecorrelation with the survival period. Conclusion The distribution density of Lc and Tc in cancer nest andperitumor interstitial tissue might be used as an immunological parameter for assessment of the prognosis.
出处
《肿瘤研究与临床》
CAS
1999年第5期294-296,共3页
Cancer Research and Clinic
关键词
食管癌
郎汉斯细胞
T细胞
预后
Esophageal carcinoma
Langerhan cell
T cell
Prognosis