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阿霉素诱导细胞凋亡过程中的信号传递 被引量:1

THE SIGNAL TRANSDUCTION OF APOPTOSIS INDUCED BY ADRIAMYCIN
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摘要 目的探讨阿霉素诱导人涎腺癌细胞凋亡的分子机制。方法通过光镜和电镜观察不同作用时间后细胞的形态学改变,DNA琼脂糖凝胶电泳,流式细胞仪分析DNA含量和细胞周期,并检测细胞内Ca(2+)浓度的变化。结果阿霉素作用于mEc-1细胞后出现了细胞凋亡的特征,细胞内Ca(2+)浓度明显增力。。结论Ca(2+)参与细胞凋亡过程可能是阿霉素诱导IEC-1细胞凋亡的机制之一。 Objective To explore whether or not driamycin inhibits mEC cells by apoptosis. MethodsAdriamycin was used for mucoepidermoid carcinoma cells mEC-l. Morphologic changes were observed atdifferent times by light and electronic microscope. DNA fragments were shown on agarose gelelectrophoresis. DNA content and cell cycles were analyzed by flow cytometer. Changes of intracellularcalcium ion was monitored by flow cytometer. Results mEC-1 cells presented with classic morphologicfeatures of apoptosis. Intracellular calcium ion was increased. Conclusion Apoptosis followed by thechanges of intracellular calcium ion in human salivary gland mucoepidermoid carcinoma cells is one of themechanisms of adriamycin inhibited tumor cell growth.
出处 《肿瘤研究与临床》 CAS 1999年第5期316-317,共2页 Cancer Research and Clinic
关键词 细胞凋亡 涎腺癌 信号传递 阿霉素 Apoptosis Human salivary gland carcinom cell lines Signal transduction
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  • 1司徒强 吴军正 等.人粘液表皮样癌细胞系MEC-1的建立及其生物特性[J].实用口腔医学杂志,1990,2:83-83.

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