基于二元混合自组装包裹纳米颗粒的微传感器抗体固定方法

Micro Biosensor Antibody Immobilization Method Based on Mixed Self-assembly-monolayers Wrapped Nano-particle

采用二元混合自组装膜修饰纳米金颗粒,在经自组装单分子层修饰的金电极上阵列式排布,并通过共价键固定抗体形成生物敏感膜。采用原子力显微镜、扫描电镜和阻抗谱分别对电极表面的修饰过程进行了表征。纳米粒子在微电极表面均匀分布,没有明显的团聚,并且可实现抗体有效固定。基于标准互补金属氧化物半导体(Complementary metal oxide semiconductor,CMOS)工艺和微加工技术,利用该抗体固定化方法,制备了糖化血红蛋白免疫微传感器,可同时检测血液中的糖化血红蛋白和血红蛋白含量,其对糖化血红蛋白和血红蛋白的检测范围分别为14～170μg/L和167～570μg/L。

Nano-gold particles that wrapped by mixed self-assembly monolayers（SAM） were immobilized on the SAM-modified Au micro electrode surface by amide linkage to form nano-particles array.Subsequently,a sensitive bio-film on the micro immunosensor surface was formed with covalent immobilization of antibodies on the nano-particles.Micro electrode surface modification process was characterized by atomic force microscopy, scanning electron microscope,and electrochemical impe-dance spectroscopy.The results indicated that the nano-particles distributed uniformly without appa-rent aggregation and the antibodies can be effectively immobilized on the microelectrode surface.Using this antibody immobilization method,hemoglobin-A1c（HbAIc） micro-sensors were prepared.Based on complementary metal-oxide-semiconductor（CMOS） standard process and micro-electronic-mechanical system（MEMS） technology,the immunosensor can detect hemoglobin-A1c and hemoglobin simultaneously with the range of 166.7-570 μg/L hemoglobin and 13.75-170.5 μg/L HbA1c.