阴离子交换树脂结合近红外漫反射光谱测定碳酸饮料中日落黄

Determination of Sunset Yellow in Carbonated Drinks by Anion Exchange Resin in Combination with Near Infrared Diffuse Reflectance Spectroscopy

采用强碱性阴离子交换树脂富集饮料中的合成食用色素日落黄,用近红外漫反射光谱技术直接测定富集有色素的树脂。将34个模拟样品建模,日落黄浓度范围为0.05～1.2 g/L。以柠檬黄和亮丽春红5R为干扰,经偏最小二乘回归建模,得到决定系数为0.9883,标准偏差为0.0187的稳健模型。定量预测3种不同市售饮料中的日落黄,回收率分别为95.1%,105.8%和99.5%。富集使样品的最小检出浓度达到9.94 mg/L。方法提高了近红外光谱技术的检测灵敏度,实现了饮料中微量日落黄的定量测定。

Synthetic colorant sunset yellow in carbonated drinks was enriched by alkaline anion exchange resin,and of the resin with adsorbate was measured directly by near infrared diffuse reflectance spectroscopy（NIDRS）.Then the calibration model was built by partial least squares（PLS） regression with 34 spiked samples and the concentrations were in the range of 0.05-1.2 g/L.The coefficient of determination（R2） and standard deviation of the calibration model were 0.9883 and 0.0187,respectively.Meanwhile,three concentrated commercial drinks with sunset yellow were used for validation and the recoveries of sunset yellow were 95.01%,105.8% and 99.54%.As a result,the trace amount of sunset yellow in commercial samples with interference was quantitatively detected and the sensitivity of near infrared spectroscopy technology was improved by this preconcentration method.