2种蜂胶总黄酮提取方法的比较

Comparison of Two Extraction Methods of Flavonoids from Propolis

[目的]为进一步开发和利用蜂胶资源提供依据。[方法]采用正交试验,对乙醇热浸法和微波辅助法提取蜂胶总黄酮的工艺和效果进行比较。[结果]乙醇热浸法提取蜂胶总黄酮的最佳工艺条件为:料液比1∶30、提取温度60℃、提取时间6 h、乙醇浓度70%,在此条件下总黄酮提取率为9.931%;微波辅助法提取蜂胶总黄酮的最佳工艺条件为:料液比1∶50、微波功率420 W、微波处理时间40 s、乙醇浓度70%,在此条件下总黄酮提取率为14.574%。[结论]微波法提取时间短、提取效率高,是一种理想的蜂胶总黄酮提取方法。

[Objective] The research aimed to provide basis for further development and utilization of propolis resources.[Method] Using orthogonal test,the process conditions and efficiency of flavonoids extraction from propolis by ethanol extraction method and microwave-assisted method were compared.[Result] The best optimum process conditions of extracting flavonoids from propolis by ethanol extraction method were as follows：solid-to-liquid ratio of 1∶ 30,extracting temperature of 60 ℃,extraction time of 6 h and ethanol concentration of 70%.Under these conditions,the extraction ratio of flavonoids was 9.931%.The optimum process conditions of extracting flavonoids from propolis by microwave-assisted extraction were as follows：solid-to-liquid ratio of 1∶ 50,microwave power of 420 W,microwave treatment time of 40 s and ethanol concentration of 70%.Under these conditions,the extraction ratio of flavonoids was 14.574%.[Conclusion] Microwave method is rapid and efficient,so it can be used as an ideal extraction method of flavonoids from propolis.