破瘀通脉散对脑缺血-再灌注损伤的影响及机制探讨

Investigations into the influences of Poyu Tongmai Powder(破瘀通脉散 ) on cerebral ischemia reperfusion injury and the mechanisms

目的 :探讨中药破瘀通脉散对脑缺血再灌注损伤的作用及可能机制。方法 :采用线栓法制作大鼠局灶性脑缺血再灌注损伤模型。将 4 5只大鼠随机分为 5组 :假手术组 ( 8只 ) ,模型组 ( 8只 ) ,治疗组中小剂量组( 9只 )、中剂量组 ( 10只 )和大剂量组 ( 10只 )。治疗组分别按体重计算 ,用中药破瘀通脉散灌胃 ,每日 1次 ,连用7日观察结果。结果 :模型组脑组织内皮素 ( ET)含量为 ( 2 1.14 4± 3 .72 6) ng/g,用药后大剂量组显著降低〔( 10 .3 68± 1.5 0 4 ) ng/g,P<0 .0 5〕,而中、小剂量组与模型组差异不显著 ( P均 >0 .0 5 )。模型组脑组织一氧化氮 ( NO)含量为 ( 13 .2 0 4± 2 .978) μmol/g,用药后中、大剂量组均显著降低〔分别为 ( 8.681± 1.85 8) μm ol/g和( 6.2 84± 0 .84 3 ) μmol/g〕,P均 <0 .0 1;与小剂量组无显著性差异 ( P>0 .0 5 )。病变程度以中、大剂量组较模型组改变显著。结论 :破瘀通脉散有抑制 ET生成的作用 ,可降低脑缺血再灌注脑组织的 NO含量 ,因此可减轻脑缺血再灌注损伤 ,其机制可能为通过调节 ET和

Objective:To investigate the actions and possible mechanisms of Poyu Tongmai Powder ( PYTMP,破瘀通脉散 ) on cerebral ischemia reperfusion injury ( CIRI) .Methods:By means of tying a thread the focal CIRI model of rats was established.4 5 m odel rats were randomly divided into five groups:sham opera- tion group ( n=8) ,model group ( n=8) ,among treatment groups small dose group ( n=9) ,m iddle dose group ( n=10 ) ,and large dose group( n=10 ) .According to the separate calculation of rat body weight in treatment group the PYTMP was poured into stom ach once a day continuously for 7days and the results were observed. Results:The concentrations of endothelin ( ET) in cerebral tissues were( 2 1.14 4± 3 .72 6) ng/ g in model group.After using drug its concentration〔( 10 .3 68± 1.5 0 4 ) ng/ g〕 in large dose group was significantly de- creased ( P<0 .0 5 ) but in comparison between middle or small dose group and m odel group the differences were not significant ( all P >0 .0 5 ) .The concentration of nitric oxide ( NO ) in brain tissues was ( 13 .2 0 4± 2 .978) μmol/ g in m odel group but after using drug it was significantly decreased in middle dose group〔( 8.681 ± 1.85 8) μm ol/ g〕and large dose group〔( 6.2 84± 0 .84 3 ) μmol/ g,all P<0 .0 1〕,furthermore compared with small dose group the difference was not significant ( P>0 .0 5 ) .The degrees of pathological changes in middle dose group and large dose group were m uch more obvious than those in model group.Conclusions:The PYTMP possesses the actions on inhibiting ET formation and is able to reduce the concentration of NO in is- chemia reperfusion brain tissues so that to relieve the CIRI.This m echanisms are possibly by m eans of regu- lating the synthesis and release of ET and NO.