固相萃取-高效液相色谱法测定植物油中的维生素E

Determination of Vitamin E in Vegetables Oils by Solid Phase Extraction-high Performance Liquid Chromatography

建立了植物油中4种维生素E的固相萃取．高效液相色谱荧光分析法。植物油中的维生素E用碱陛氧化铝萃取小柱萃取和净化后用高效液相色谱法测定，在0．1-25mg／L的浓度范围内线性关系良好，相关系数范围为0．9991-9996，方法检测限为0．0194）．033mg／kg；用3种植物油作添加回收率和精密度实验，回收率在88．1-99．2％之间，变异系数为1．39-3．95％。实验结果表明，该方法操作简便，结果准确可靠。

A solid phase extraction-high performance liquid chromatographic （SPE-HPLC） method was developed for the simultaneous analysis of four kinds of vitamin E in the vegetables oils. The vitamin E in the vegetables oils was extracted and purified by Alumina B SPE column. After the solid phase extraction, the purified extract was analyzed directly by high performance liquid chromatography with fluorescence detector. An excellent linearity was obtained in the range of 0.1-25 mg/L with the correlation coefficient （r） fi＇om 0.9991 to 0.9996; the limit of detection was in the range of 0.019~0.033 mg/kg; the mean extraction recoveries were in the range of 88.1-99.2% with the relative standard deviations （RSD） from 1.39 % to 3.95 % in three different vegetables oils matrixes. It was a simple and rapid method with satisfactory repeatability and recovery to determinate Vitamin E in vegetable Oils.