摘要
应用计算熔解温度的方法,探索应用该方法设计新型基因探针的可能性.利用荧光检测技术进行靶DNA与错配DNA链的鉴别,从而研究了DNA熔解温度对设计新型基因探针的影响.
A research on the possibility to design novel probes by using the method of calculating melting temperature has been done.The authors of this paper use fluorescence detection to identity the target and one-base mismatched DNA.By doing this,the authors want to study the influence of melting temperature on the design of novel probes.
出处
《常熟理工学院学报》
2011年第4期56-60,共5页
Journal of Changshu Institute of Technology
基金
国家自然科学基金(81001686)资助项目
关键词
熔解温度
基因探针
荧光检测
melting temperature（Tm）
probe
fluorescence detection
