摘要
目的:建立制八角枫中八角枫碱的含量测定方法。方法:色谱柱采用Alltech Apollo C18(4.6 mm×250 mm,5μm),以乙腈-缓冲液(0.2 g庚烷磺酸钠、3.5 g磷酸二氢钾定容至1 000 mL)(9∶91)为流动相,流速1.0 mL·min-1,检测波长259 nm,柱温30℃。结果:八角枫碱在0.024 95～0.499 g·L-1线性关系良好(r=0.999 9,n=5),平均回收率98.73%(n=6),RSD 0.915%。结论:方法简便、快速、准确,重复性好,专属性强,可作为制八角枫中八角枫碱的含量测定方法,以控制制八角枫的质量。
Objective：To establish a method for determination of Anabasine in Radix Alangii Preparata by HPLC.Method：The chromatographic column was Alltech Apollo C18（4.6 mm×250 mm,5 μm）,acetonitrile-buffer（0.2 g heptane sulfonate,3.5 g potassium dihydrogen phosphate volume to 1 000 mL）（9∶91） as mobile phase,flow rate 1.0 mL·min-1;detection wavelength was at 259 nm;column temperature was at 30 ℃.Result：Maple octagonal base was linear in range of 0.024 95-0.499 g·L-1（r=0.999 9,n=5）.The average recovery was 98.73%（n=6）,RSD 0.915%.Conclusion：The method is simple,rapid,accurate,reproducible,specific,and can be used as the system in the Radix Alangii Preparata method for the determination of Anabasine to control the quality of Radix Alangii Preparata.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第12期56-58,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家"十一五"科技支撑计划项目(2007BAI48B08-5)
