HPLC同步测定克咳胶囊中麻黄碱、吗啡

Simultaneous Determination of Ephedrine Hydrochloride andMorphine in Keke Capsule by HPLC

目的:建立克咳胶囊中麻黄碱、吗啡含量的高效液相色谱同时测定含量的方法。方法:采用高效液相色谱法,色谱柱依利特SinoChrom ODS-BP C18(4.6 mm×250 mm,5μm),流动相为乙腈-0.01 mol·L-1磷酸二氢钾溶液-磷酸-三乙胺(5∶95∶0.2∶0.4),检测波长222 nm,柱温为室温,流速1.0 mL·min-1,进样量10μL。结果:盐酸麻黄碱、吗啡的线性范围分别为0.16～0.8 g.L-1(r1=0.999 6),0.012～0.06 g·L-1(r2=0.999 1),线性良好,平均加样回收率分别为96.13%,95.26%,RSD分别为1.10%,1.60%。结论:该法快速、简便、重复性好,可用于克咳胶囊的质量评价。

Objective：To establish an HPLC method for simultaneous determination of ephedrine hydrochloride and morphine in Keke capsule.Method：The analysis carried out on a Sino Chrom ODS-BP C18（4.6 mm×250 mm,5 μm）.Mobile phase comprised with methyl cyanides,0.01 mol·L-1 potassium dihydrogen phosphate,phosphoric acid and triethanolamine under a ratio as 5∶95∶0.2∶0.4.Detect wave-length was at 222 nm.Flow rate was 1.0 mL·min-1under room column temperature.Sample size was 10 μL.Result：The calibration curves were linear over the range of 0.16-0.8 g·L-1 for ephedrine hydrochloride and 0.012-0.06 g·L-1 for morphine respectively.The average recoveries were 99.09% for ephedrine hydrochloride with RSD 1.24% and 98.44% for morphine with RSD 1.45% respectively.Conclusion：The determination method is rapid,simple,and repeatable,and can be used for the quality control of Keke capsule.