HPLC测定芩百清肺浓缩丸中次黄嘌呤

Determination of Hypoxanthine in Qinbai Qingfei Concentrated Pills by HPLC

目的:建立芩百清肺浓缩丸中次黄嘌呤的高效液相含量测定法。方法:采用HPLC-DAD法,色谱柱Diamonsil C18(4.6 mm×250 mm,5μm);流动相甲醇-水(2∶98),检测波长254 nm,流速1.0 mL·min-1,柱温30℃。结果:次黄嘌呤在1.94～31.04μg呈良好线性关系,r=0.999 9,平均回收率为99.14%,RSD 1.28%。结论:方法操作简便、灵敏度高、准确性和重复性好,可作为芩百清肺浓缩丸的质量控制方法。

Objective：To establish the HPLC-DAD method for determination of the Hypoxanthine in Qinbaiqingfeinongsuo pills.Method：The separation was performed in a Diamonsil C18（4.6 mm×250 mm,5 μm） column with a mobile phase of methanol-water（2∶98） in a flow rate of 1.0 mL·min-1 elution at 254 nm and the column temperature was 30 ℃.Result：The linear range of Hypoxanthine was 1.94-31.04 mg·L-1（r=0.999 9）.The average recovery was 99.14%,RSD 1.28%.Conclusion：This method is simple,reliable,accurate,which offers advantages of higher sensitivity and precision.It can be used for quality control of Qinbai qingfei nongsuo pills.