基于药效物质基础的肉桂和赤石脂相畏研究

Based on Pharmacodynamic Material Base of Cinnamon and Red Halloysite Phase Fear Research

目的:采用HPLC测定肉桂单煎液和肉桂与赤石脂不同比例配伍合煎液的水提液和挥发油中肉桂酸和肉桂醛的含量,从药效物质基础变化研究赤石脂对肉桂的相畏作用。方法:取肉桂,肉桂1∶1配伍赤石脂和肉桂1∶3配伍赤石脂3组药材,采用加热回流提取和挥发油提取法同时提取,同时收集水提液和挥发油两组成分,对提取成分采用HPLC分析。采用Phenomenex-C18色谱柱(4.6 mm×250 mm,5μm),柱温30℃,流速1.0 mL·min-1,检测波长254 nm,流动相乙腈(A)-0.1%磷酸(B),二元线性梯度洗脱0～30 min,90%B～65%B(10%A～35%A);30～50 min,65%B(35%A)的条件,测定水提液中肉桂酸和肉桂醛的含量。采用Phenomenex-C18色谱柱(4.6 mm×250 mm,5μm),柱温30℃,流速1.0 mL·min-1,检测波长280 nm,流动相乙腈(A)-0.1%磷酸(B),0～30 min,65%B(35%A)的条件,测定挥发油中肉桂醛的含量。结果:肉桂1∶1配伍赤石脂及肉桂1∶3配伍赤石脂水提液中肉桂酸的含量照单煎肉桂药材分别下降30.71%,53.54%,未检测到肉桂醛。挥发油中肉桂醛含量分别下降7.21%,75.89%。结论:肉桂与赤石脂配伍后肉桂酸和肉桂醛含量均下降,且下降程度与赤石脂比例有关。

Objective：Determined by HPLC method cinnamon single Fried fluid and cinnamon and red halloysite different proportion of gegenqinlian decoction of liquid water extraction fits the liquid and naphtha in cinnamic acid and cinnamaldehyde content,material basis from the pharmacodynamic changes of Chishizhi Wei on the role of the phase of cinnamon.Method：Take the cinnamon,cinnamon drew red halloysite and cinnamon compatibility of purified red halloysite three groups of compatibility of medicinal herbs,adopt heating reflux extraction and volatile oil extraction meanwhile extraction and collected water extraction liquid and naphtha two composition points,to extract use HPLC analysis.Adopt chromatographic column：Phenomenex-C18（4.6 mm×250 mm,5 μm）,column temperature 30 ℃,velocity was 1.0 mL·min-1,detected wavelength was 254 nm,mobile phase was acetonitrile（A）-0.1% phosphate（B）,binary linear gradient elution 0-30 min,90% B-65% B（10% A-35% A）;30-50 min,65% B（35% A）conditions,determination of water extraction fluid cinnamic acid and cinnamaldehyde content.Adopt chromatographic column：Phenomenex-C18（4.6 mm×250 mm,5 μm）,column temperature was 30 ℃.Velocity was 1.0 mL·min-1,detected wavelength was 280 nm,mobile phase was acetonitrile（A）-0.1% phosphate（B）,0-30 min,65% B（35% A）conditions,determination of cinnamaldehyde content of volatile oil.Result：Cinnamon drew compatibility red halloysite and cinnamon purified water extract-alcohol compatibility red halloysite in liquid cinnamic acid content of single Fried cinnamon herbs according to drop respectively 30.71%,53.54%,cinnamaldehyde not detected.Volatile oil of cinnamaldehyde content decreased respectively 7.21%,75.89%.Conclusion：Cinnamon and red halloysite after cinnamic acid and compatibility of cinnamaldehyde content decreased,and drop extent with relevant red halloysite percentage.