冬虫夏草提取物调血脂与抗氧化活性

Cordyceps Militaris Extracts on Blood Lipid Regulation in vivo and Antioxidation in vitro

目的:研究冬虫夏草提取物(YCC)在调血脂与抗氧化方面的活性。方法:建立高脂饮食诱导的高脂血症金黄地鼠模型,连续ig给予200 mg·kg-1的YCC 4周后,比色法测定动物血清中总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-c)与高密度脂蛋白胆固醇(HDL-c)的含量,观察YCC调节脂质代谢的活性;基于体外分子水平反应体系,运用比色、荧光、化学发光的方法评价不同浓度YCC对DPPH、超氧阴离子、羟自由基、脂质过氧自由基、过氧化氢及过氧亚硝基的清除活性;建立铜离子诱导人低密度脂蛋白(LDL)氧化修饰模型,并观察YCC对LDL氧化过程的影响。结果:与模型组比较,YCC在200 mg·kg-1剂量下明显降低了高脂饮食饲养的地鼠血清中TC,TG,LDL-c含量,同时升高HDL-c/LDL-c(P<0.05);YCC对1,1-二苯基-2-苦肼基自由基(DPPH)和过氧化氢的半数抑制浓度(IC50)分别为(125.6±3.4)mg·L-1,(758.9±11.2)mg·L-1;YCC对脂质过氧自由基、过氧亚硝基、羟自由基的最大清除率分别为(86.5±14.5)%,(64.4±3.3)%,(44.1±6.0)%;YCC在50,100 mg·L-1浓度下可明显延长铜离子诱导LDL氧化过程的滞留时相。结论:YCC在体内显示出调血脂、体外显示出抗氧化活性。

Objective：To investigate the effects of Cordyceps militaris extracts（YCC） on regulating serum lipids and antioxidation.Method：We established high-fat diet-induced hyperlipidemic golden hamster model.After the four-week administration of YCC（200 mg·kg-1）,the levels of total cholesterol（TC）,triglyceride（TG）,low density lipoprotein-cholesterol（LDL-c）,and high density lipoprotein-cholesterol（HDL-c） in serum were assayed with the method of chromatometry to observe the effects of YCC on serum lipid regulation;we also evaluated the scavenging activity of YCC against a array of free radicals,including 1,1-diphenyl-2-picrylhydrazyl radical（DPPH）,superoxide anion,hydroxyl radical,peroxyl radical,hydrogen peroxide and peroxynitrite based on molecular level reactions in vitro;at the same time,we evaluated the effect of YCC on cupric ion-induced human low density lipoprotein（LDL） oxidation.Results：Compared with model group,YCC decreased the levels of serum TC,TG,LDL-c significantly,while increased the ratio of HDL-c/LDL-c（P0.05） at the dose of 200 mg·kg-1.The IC50 of YCC against DPPH and hydrogen peroxide are（125.6±3.4） mg·L-1,（758.9±11.2） mg·L-1 respectively.The maximal scavenging activity of YCC against peroxyl radical,peroxynitrite and hydroxyl radical is（86.5±14.5）%,（64.4±3.3）%,（44.1±6.0）%.In addition,YCC prolonged the lag phase of LDL oxidation process at 50,100 mg·L-1.Conclusion：YCC displayed serum lipid regulating in vivo and antioxidative activity in vitro.