常春卫矛及爬行卫矛提取物止血消瘀药效研究

Pharmacodynamic Study on Removing Blood Stasis and Stopping Bleeding for Extracts of Euonymus fortunei and Euenymus hederaceus

目的:研究常春卫矛提取物(EH)和爬行卫矛提取物(EF)的止血消瘀作用。方法:将小鼠随机分成8组,分别设EH和EF的高、中、低剂量(32.0,16.0,8.0 g·kg-1)组,阿斯匹林(100 mg·kg-1)组和空白组,ig,采用毛细玻璃管法测量小鼠凝血时间;将小鼠随机分为9组,分别设EH和EF的高、中、低剂量组,空白组,模型组,复方扶芳藤合剂(百年乐)(5.2 g·kg-1)组,建立血虚小鼠模型,观察小鼠WBC,RBC,Hb的变化;将大鼠随机分成9组,分别设EH和EF的高、中、低剂量组,空白组,模型组,复方丹参片(0.6 g·kg-1)组,ig,建立急性血瘀证大鼠模型,观察EH和EF对大鼠血液流变学指标的影响。结果:EH高、中剂量组和EF高剂量组能显著延长凝血时间;百年乐及EF高、中剂量组对WBC,RBC数及Hb有明显增加趋势;EH高、中剂量组能明显降低急性"血瘀"证大鼠全血黏度和血浆黏度,红细胞刚性指数,高剂量组还能降低红细胞压积、血沉和TC、TG含量,EF高、中剂量组降低全血黏度和血浆黏度、血沉、全血还原黏度,高剂量还能降低红细胞刚性指数、红细胞聚集指数、红细胞压积及TG含量。结论:EH和EF均具有抗凝血和活血化瘀作用。

Objective：To investigate the effects of Euonymus fortunei.extract（EF） and E.hederaceus extract（EH） on removing blood stasis and stopping bleeding.Method：Kunming mice were randomly divided into eight groups：EH and EF treatment（32.0,16.0,8.0 g·kg-1,ig） groups,aspirin（100 mg·kg-1,ig） group and control group,ig,the anticoagulant effect of EH and EF was observed by measuring blood coagulation time in mice with capillary tube method.In another study,Kunming mice randomly divided into nine groups：EH and EF treatment（32.0,16.0,8.0 g·kg-1,ig） groups,Fufang Fufangteng mixture（bainianle）（5.2 g·kg-1,ig）group,model group and control group,to observe the changes of WBC,RBC,Hb level.Wistar rats were randomly divided into nine groups：EH and EF treatment（32.0,16.0,8.0 g·kg-1,ig） groups,Fufang Dansen tablet（0.6 g·kg-1,ig）group,model group and control group.Rat model of acute blood stasis syndrome was established to observe the influence of EF and EH on hemorheological parameters.Result：Under the doses of EH（32.0,16.0 g·kg-1） and EF（32.0 g·kg-1）,the experiments showed that EH and EF could significantly extend the clotting time;Under the doses of EF（32.0,16.0 g·kg-1）,EF and bainianle could increase the WBC,RBC count and HB value significantly in hemopenia mice;Under the doses of EH（32.0,16.0 g·kg-1）,EH could reduce the parameters of hemorheology in rats with acute blood stasis syndrome,including whole blood viscosity,plasma viscosity,rigidity index of RBC,and EH（32.0 g·kg-1） could reduce HCT,erythrocyte sedimentation rate（ESR）,the content of TC and TG.Under the doses of EF（32.0,16.0 g·kg-1）,EF could reduce the parameters of hemorheology in rats with acute blood stasis syndrome,including whole blood viscosity,plasma viscosity,ESR,whole blood reduced viscosity,and EF（32.0 g·kg-1） could reduce rigidity index of RBC,aggregation index of RBC,HCT,ESR and the content of TG.Conclusion：Both extracts have the effects of activationg blood circulation and removing blood stasis.