香连丸有效部位对小鼠急性溃疡性结肠炎的治疗作用

Effect of Effective Fraction of Xianglian Pill on Acute Ulcerative Colitis in Mice

目的:研究香连丸有效部位对葡聚糖硫酸钠(dextran sulfate sodium,DSS)和20%乙醇联合诱导小鼠急性期溃疡性结肠炎(ulcerative colitis,UC)的治疗作用。方法:建立DSS和20%乙醇联合诱导的小鼠急性期UC模型,ig给予香连丸有效部位(0.292,0.584,1.168 g·kg-1),观察各组小鼠的一般状态、疾病活动指数、结肠性状、结肠组织病理学变化,进行疾病活动指数评分(disease activity index,DAI)、测定各鼠结肠长度质量比、脾脏系数、血清中丙二醛(MDA)的含量。结果:香连丸有效部位能显著改善结肠炎小鼠的一般状态,改善结肠的性状,减轻其结肠组织病理学损伤,降低其血清中MDA的含量。结论:香连丸有效部位对小鼠溃疡性结肠炎有较好的治疗作用。

Objective：To investigate the therapeutic effects of effective fraction of Xianglian Pill on acute ulcerative colitis（UC） induced by dextran sulfate sodium（DSS） and 20% alcohol in mice.Method：Acute ulcerative colitis was induced by DSS and 20% alcohol,and effective fractions of Xianglian Pill were given by stomach for 7 days at the same time.The genera situations and histopathological changes were observed during the experiment disease activity index（DAI） and colon length,intestinal weight index,spleen weight and The levels of serum MDA were evaluated.Result：Effective fraction of Xianglian Pill greatly ameliorated the symptom of UC and histopathological changes as compared with UC control.The levels of serum MDA were obviously reduced.Conclusion：Effective fraction of Xianglian Pill is much helpful to the colitis mouse induced by DDS and 20% alcohol.