四逆汤对血管性痴呆大鼠学习记忆力的影响

Effect of Sini Decoction on Learning and Memory Ability in Rats with Vascular Dementia

目的:探讨四逆汤(SND)对血管性痴呆(VD)大鼠学习记忆能力的影响及作用机制。方法:将大鼠随机分为假手术组、VD模型组和SND组,ip硝普钠后反复夹闭及再通双侧颈总动脉复制VD大鼠模型,SND组给予四逆汤(3.5 g·kg-1)ig28 d。Y型电迷宫和水迷宫检测大鼠学习记忆能力,测定脑组织中一氧化氮合酶(NOS)、一氧化氮(NO)和谷胱甘肽过氧化物酶(GSH-Px)的含量。结果:与假手术组比较,VD模型组大鼠错误反应次数明显增多,全天总反应时间明显延长(P<0.05),逃避潜伏期和总路程明显延长(P<0.05),脑组织NOS及NO含量升高(P<0.05),GSH-Px活性下降(P<0.05)。与VD模型组比较,SND能提高脑组织GSH-Px活性(P<0.01),降低NOS及NO含量(P<0.01),大鼠错误反应次数明显减少,全天总反应时间明显缩短(P<0.05),潜伏期和总路程均缩短(P<0.01)。结论:SND可以改善VD大鼠学习记忆能力,其机制可能与增强抗氧化能力有关。

Objective：To explore the effect of Sini Decoction（SND） on learning and memory ability of vascular dementia（VD） in rats and the related mechanism.Method：VD model of rat was established by repeated cerebral ischemia/reperfusion after the decrease in blood pressure induced by sodium nitroprusside.Thirty rats were randomly divided into Sham-operated group;VD model group and SND group（3.5 g·kg-1,ig 28 d）.The learning and memory ability was tested with Y-maze and water maze.The production of NOS,NO and glutathione peroxidase（GSH-Px） in cerebral cortex and hippocampus was detected.Result：Learning and memory abilities were decreased,NOS and NO were increased,the activities of GSH-Px were reduced in VD group,compared with those in the sham-operated group（P0.05）.In SND group,the learning and memory ability was increased,NOS and NO were decreased and the activity of GSH-Px was increased,comparedwith those in the VD group（P0.01）.Conclusion：These findings suggested that SND could improve the learning and memory ability in VD rats,which may be due to the increased anti-oxidative ability of brain tissue.