养阴固精化痰渗湿方联合贝那普利对早期糖尿病肾病大鼠的保护作用

Protect Effect of Combinded Therapy of Herbal and Benazepril on Early Stage of Diabetic Nephropathy in Rats

目的:探讨中药养阴固精化痰渗湿方联合贝那普利对早期糖尿病肾病(DN)大鼠的疗效及其作用机制。方法:雄性Wistar大鼠随机分为正常组(ip缓冲液)和造模组(ip STZ)。大鼠成模后随机分为模型组及中药+贝那普利组,正常组和模型组以蒸馏水ig,中药(含生药50 g·kg-1)+贝那普利(5 mg·kg-1)组给予药物8周后处死动物。HE,PAS染色观察肾组织病变程度,并用免疫组织细胞化学方法分析大鼠肾组织中转化生长因子-β1(TGF-β1)、Ⅳ型胶原蛋白(CollagenⅣ)、血管紧张素Ⅱ(AngⅡ)和单核/巨噬细胞(ED-1)的表达情况。结果:中药+贝那普利组大鼠肾小球毛细血管基底膜增厚及肾小球系膜细胞增生、系膜基质增多、系膜区增宽等现象较模型组明显减轻;中药+贝那普利组大鼠肾组织中TGF-β1,CollagenⅣ,AngⅡ,ED-1的表达量较模型组下降,差异有统计学意义(P<0.01)。结论:养阴固精渗湿方联合贝那普利能有效减轻DN大鼠的病理损害,延缓其进程。

Objective：To study the effect and mechanism of combined Chinese and Western medicines in early diabetic nephropathy（DN） in rats.Method：The male wistar rats were randomly divided into 2 groups：normal and DN groups.Experimental DN was induced in DN group with streptozotocin（STZ）,and the normal group was given equal volume of distilled water.Then the modeled rats were subrandominzed into model group and benazepril（5 mg·kg-1）＋herbal group（50 g·kg-1）.Benazepril＋herbal group was medicated respectly,the other two groups were given distilled water.After the rats had been treated for 8 weeks,transforming growth factor-β1（TGF-β1）,collagen Ⅳ,angiotensinⅡ（AngⅡ）,monocyte/macrophage（ED-1） in the renal tissues of the rats were detected by immunohistocytochemistry.Result：Compared with model group,the thickened glomerular basement membrane,increased mesangial cells,proliferated mesangialmatrix,and enlarged mesangial area became lightened in benazepril＋herbal group after treatment.Moreover,TGF-β1,collagen Ⅳ,AngⅡ and ED-1 were reduced when compare to model group,but which were higher than normal group.Conclusion：This recipe combined with benazepril can reduce the pathological damage of early DN and postphone the development of DN.