结肠癌手术对肠道菌群微生态的影响

The impact of colon cancer surgery on the gut microflora

目的观察结肠癌手术处理对肠道菌群微生态的影响。方法收集手术前后结肠癌患者的粪便标本60份,采用实时荧光定量PCR法检测肠道拟杆菌属、梭杆菌属及梭菌属量的变化。结果手术前后粪便中细菌数量分别为:拟杆菌属(9.64±0.58 vs.6.21±0.37),梭杆菌属(9.87±0.72 vs.6.36±0.68),梭菌属(7.01±0.37 vs.5.30±0.29);拟杆菌属中的脆弱拟杆菌为(5.92±0.24 vs.3.24±0.78),梭杆菌属中的坏死梭杆菌为(7.12±0.97 vs.3.70±1.21),梭菌属中的肉毒梭菌为(5.68±0.36 vs.1.52±1.06),艰难梭菌为(3.45±0.38 vs.1.42±0.25)。术后患者粪便中细菌数量明显低于术前,P均<0.05。结论结肠癌患者粪便中拟杆菌属、梭杆菌属和梭菌属术后数量较术前明显降低,提示手术对肠道菌群有一定抑制。

Objective To observe the impact of colon cancer surgery on the gut microflora. Methods Collected the feces of patients who underwent colon cancer resection （ n = 60）. Real-time fluorescence quantitative PCR was used to analyze the changes of the number of Bacteroides, Fusobacterium, Clostridium. Results The preoperative level of Bacteroides（ 9. 64 ±0.58 vs. 6.21 ±0.37 ） , Fusobacterium （9.87 ±0.72 vs. 6.36± 0.68）, Clostridium（ 7. 01 ±0.37 vs. 5.30±0.29）, B.fragilis （5.92 ±0.24 vs. 3.24 ±0.78）, F. necrosis （7.12 ± 0.97 vs. 3.70 ± 1.21 ）, C. botulinum （5.68 ± 0.36 vs. 1.52 ± 1.06） and C. difficile （ 3.45 ± 0.38 vs. 1.42 ±0.25 ） were significantly higher than those of postoperative （ P 〈 0.05 ）. Conclusions The fecal Bacteroides, Fusobacterium and Clostridium composition of colon cancer patients are significantly lower than those of preoperative group. Surgery in patients of colon cancer has a certain influence on the intestinal flora.