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牙龈卟啉单胞菌及其细胞外膜泡多克隆抗体的比较研究 被引量:1

COMPARISON OF THE TWO KINDS OF POLYCLONAL ANTIBODY OF PORPHYROMONAS GINGIVALIS
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摘要 分别制备牙龈卟啉单胞菌47A- 1 及其膜泡的多克隆抗体,并用间接免疫荧光法对67 个临床标本进行检测。结果经统计学检验,两种抗体显著相关,P< 0.01 ,符合率为82.1 % ;膜泡抗体的检出率高于菌体抗体,P<0.05 ;膜泡抗体相对于菌体抗体的敏感性为93.5 % 。膜泡抗体与牙龈卟啉单胞菌以外的其他口腔细菌无交叉反应,说明膜泡抗体的特异性较强。 In the present study,we prepared the polyclonal antibody of P.g and the polyclonal antibody of ECV,and compared the two antibodies using indirect immunofluorescence assay to 67 clinical samples.The two kinds of antibody are significant correlated(p<0.01), the accuracy is 82.1%.The positive rate of ECV antibody is higher than that of P.g antibody (p<0.05).The sensitivity of ECV antibody compared to P.g antibody is 93.5%.The specificity of ECV antibody is very well because it does not cross-react with other oral strains.
出处 《中国微生态学杂志》 CAS CSCD 1999年第5期312-313,共2页 Chinese Journal of Microecology
关键词 牙龈卟啉单胞菌 细胞外膜泡 多克隆抗体 Porphyromonas gingivalis Polyclonal antibody
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参考文献3

  • 1薛毅,中国微生态学杂志,1993年,5期,31页
  • 2吉重华,中华口腔医学杂志,1991年,26卷,354页
  • 3李成文,现代免疫化学技术,1990年,149页

同被引文献8

  • 1Lamont RJ,Jenkinson HF.Life below the gum line:Pathogenic mechanisms of Porphyromonas gingivalis MMBR,1998,62 (4):1092-2172.
  • 2Schenkein HA,Hansel M,Fletcher HM,et al.Increased opsonization of a prtH-defective mutant of Porphyromonas gingivalis W83 is aused by reduced degradation of complement-derived opsonins.J Immunol,1995,154:5331-5337.
  • 3Fletcher HM,Schenkein HA,Macrina FL.Cloning and characterization of a new protease gene (prtH) from Porphyromonas gingivalis.Infect Immun,1994,62 (10):4279 -4286.
  • 4Fletcher HM,Schenkein HA,Morgan RM,et al.Virulence of a Porphyromonas gingivalis W83 mutant defective in the prtH gene.Infect Immun,1995,63(4):1521-1528.
  • 5Nakagawa T,Sims T,Fan Q,et al.Functional characteristics of antibodies induced by Arg-gingipain (RgpA) and Lys-gingipain (Kgp) from Porphyromonas gingivalis.Oral Microbiol Immunol,2001,16:202-211.
  • 6Slakeski N,Margetts M,Moore C,et al.Characterization and expression of a novel Porphyromonas gingivalis outer membrane protein,Omp28.Oral Microbiol Immunol,2002,17(3):150-156.
  • 7Potempa J,Pike R,Travis J.The mutiple forms of trypsin-like activity present in various strains of Porphyromonas gingivalis are due to the presence of either Arg-gingipain or Lya-gingipain.Infet Immun,1995,63:1176-1182.
  • 8Yonezawa H,Ishihara K,Okuda K.Arg-Gingipain A DNA vaccine induces protective immunity against infection by Porphyromonas gingivalis in a murine model.Infect Immun,2001,69(5):2858-2864.

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