慢性甲基苯丙胺中毒大鼠和人血清中硝基化损伤水平及其神经毒性的研究

RESEARCH OF NITRATION MODIFICATION IN CHRONIC METHAMPHETAMINE TREATED RATS AND HUMAN SERUM AND THE NEUROTOXICITY

目的:通过观察慢性甲基苯丙胺中毒大鼠及长期甲基苯丙胺依赖人类血清中3-NT的含量变化,间接反映体内ONOO-的含量变化,探讨甲基苯丙胺导致蛋白质硝基化参与的神经毒性机制。方法:建立慢性甲基苯丙胺中毒大鼠模型,收集甲基苯丙胺长期依赖者血清与未吸毒的正常人血清;ELISA检测大鼠及人血清内3-NT的含量。结果:与对照组相比,甲基苯丙胺组大鼠和人血清中3-NT含量明显增多,差异有统计学意义(P<0.001)。结论:慢性甲基苯丙胺中毒动物和长期毒品依赖者血清的3-NT含量增高,表明其所代表的关键蛋白的硝基化参与了甲基苯丙胺的神经毒性作用。

Objective：To study the changes of 3-NT in the rats and human＇s blood serum,and then indirectly reflect the ONOO-content in vivo to further research methamphetamine（METH）-induced nitroprotein in the pathogenesis of METH neurotoxicity.Methods：Forty rats were randomly divided into two groups for injections with METH（n=20） or saline（n=20）;and collect the human＇s serum who were addicted with METH and not.ELISA was used to measure the changes in the rats and human＇s blood serum.Results：The 3-NT content was significantly increased in the blood serum of rats and human with METH treatment as compared with those in the saline group（P0.001）.Conclusion：Increased 3-NT content in rats and human shows that the nitration of vital structure proteins in vivo may be a key pathway in METH-induced neurotoxicity.