摘要
目的:通过对醉酒人群的CYP2E1基因的检测,获得该基因遗传多态性数据,及其在醉酒人群中的分布。方法:采用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)技术,检测CYP2E1基因在154例醉酒样本的等位基因、基因型、基因频率。结果:CYP2E1基因在154例醉酒样本中共检出两种等位基因:等位基因c1和c2,观察到3种基因型c1/c1、c1/c2和c2/c2,基因型频率分别是68.8%、29.2%和1.9%。结论:对醉酒人群的CYP2E1基因的检测实验结果与本课题组先前的研究结果(酒依赖和健康对照组CYP2E1基因多态性)做统计分析。统计结果提示:醉酒人群的CYP2E1基因分布和酒依赖组没有差别,而与健康人群的CYP2E1基因分布存在差异,并且差异有统计学意义(P<0.05)。本研究用饮酒量分组与基因型分组,做统计分析,结果没有统计学意义。
Objective：To obtain the genetic polymorphism data and the distribution by testing drunkenness CYP2E1 gene.Methods：The PCR-RFLP was used to analyze genetic polymorphism of CYP2E1 gene and test the genotype and gene frequencies in 154 cases.Results：Two alleles were detected in the CYP2E1 gene of 154 drunkenness samples：alleles c1 and c2,including three genotypes c1/c1、c1/c2 and c2/c2,the total of which were the 68.8%,29.2% and 1.9%,respectively.Conclusion：The results of the statistical analysis of this experiment and previous studies（alcohol dependence and healthy control group CYP2EI polymorphism）suggest：there is no different CYP2E1 gene distribution between drunkenness group and alcohol dependence group but significant difference exists when compared with the healthy control group（P0.05）.The results of the statistical analysis by alcohol consumption and genotype indicate no statistical significance.
出处
《中国药物依赖性杂志》
CAS
CSCD
2011年第3期190-194,共5页
Chinese Journal of Drug Dependence
基金
昆明医学院研究生创新基金资助(KM2009J11)
