提高疣粒野生稻愈伤组织分化能力的研究

Improvement of Plant Differentiation Frequency of Wild Rice Oryza Meyeriana calli

通过低温处理、更换培养基凝固剂以及液固交替培养的方式，成功地保持了疣粒野生稻愈伤组织的胚性，提高了绿苗分化率。结果表明：低温处理１２ 天的愈伤组织绿苗分化率最高；用琼脂糖代替琼脂作为培养基凝固剂，能在较大程度上提高愈伤组织绿苗分化率；通过液固交替培养的方式保持了悬浮系的分化潜能，在悬浮培养１８ 个月后，绿苗分化率仍达到４１ ．６ ％ ，而持续悬浮培养１２ 个月的愈伤组织全部丧失分化能力。

To increase plant differentiation frequency, calli of wild rice Oryza meyeriana were treated in different methods. Main results are showed as follows. (1)Low temperature at 4 8℃ promoted plant differentiation frequency of long term calli cultures. The effect was most prominent for 12 day's treatment. (2) Agarose in place of agar as gelling agent in medium significantly increased green plant differentiation frequency. (3) Liquid solid alternation subculture was proved as an effective method to maintain embryogenic property of the long term cultured calli. Green plant differentiation frequency was kept above 41.6% after 18 month alternation culture, while plant differentiation ability was lost after 12 month continuous suspension culture. (4) Variation of esterase isozyme pattern showed that some isozymes bands correlated with the differentiation ability of calli of O. meyeriana.