乌司他丁对大鼠肢体缺血再灌注时肺组织NF-κB表达的影响

Effects of ulinastatin on expression of NF-κB in lung tissues during limb ischemia-reperfusion in rats

目的探讨乌司他丁对大鼠肢体缺血再灌注时肺组织NF-κB表达的影响。方法雄性SD大鼠48只，体重230—260g，采用随机数字表，将其随机分为3组（n=16）：假手术组（S组）、肢体缺血再灌注组（I／R组）和乌司他丁组（u组）。I／R组和u组采用夹闭双侧股动脉2h再开放的方法制备后肢缺血再灌注诱发肺损伤模型。分别于再灌注2、4h时处死8只大鼠，取肺组织，计算肺组织湿／干重比，采用免疫组化法测定NF-xB表达水平，光镜下观察病理学结果。结果与S组比较，I／R组肺组织湿／干重比升高，肺组织NF--κB表达上调（P〈0．05）；与I／R组比较，U组肺组织湿／干重比降低，肺组织NF—κB表达下调（P〈0．05）。u组肺组织损伤程度轻于I／R组。结论乌司他丁可下调肺组织NF-κB表达，从而减轻大鼠肢体缺血再灌注诱发肺损伤。

Objective To investigate the effects of ulinastatin on the expression of NF-κB in lung tissues during limb ischemia-reperfusion （I/R） in rats. Methods Forty-eight male SD rats weighing 230-260 g were randomly divided into 3 groups （n = 16 each） ： sham operation group （group S） ; I/R group; ulinastatin group （group U）. A rat model of lung injury induced by I/R of hind limbs which was produced by occlusion of the bilateral femoral arteries for 2 h followed by reperfusion was established. The rats were sacrificed at 2 and 4 h of reperfusion （8 rats at each time point） and the lung tissues removed for determination of NF-κB expression （by immuno-histo- chemistry） and microscopic examination. W/D lung weight ratio was calculated. Results W/D lung weight ratio was significantly increased and NF-κB expression was up-regulated in group I/R compared with group S （P 〈 0.05）. W/D lung weight ratio was significantly decreased and NF-κB expression was down-regulated in group U compared with group I/R （P 〈 0.05）. The lung injury induced by I/R was reduced in group U compared with group I/R. Conclusion Ulinastatin can reduce the lung injury induced by limb I/R by down-regulating the expression of NF-κB in rat lung tissues.