全期表达外源基因的重组杆状病毒

WHOLE PHASE EXPRESSION OF FOREIGN GENE IN A RECOMBINANT BACULOVIRUS

构建了含早期、晚期、极晚期启动子、全期表达ＨＢｓＡｇ 基因的重组杆状病毒ｖＴｎＮＰＶＰ３５ＨＢｓＯＣＣ＋ ．结果ＨＢｓＡｇ 表达时间由感染后１２ ｈ 提前到４ ｈ，而其表达量，特别是病毒感染早期的表达水平亦显著提高，其中２４ ｈ 和９６ ｈ 的表达量分别比对照提高１８０ ％ 和６０ ％ ． 转录起始位点分析结果显示，该重组毒株可在病毒感染的早。

A recombinant baculovirus vTnNPV P 35 HBs OCC + was constructed, containing the HBsAg gene with the control of the early, late, and very late promoters. The expression products of HBsAg gene could be detected at 4 h postinfection. The expression level of HBsAg was increased by 180% and 60% compared with the control at 24 h and 96 h, respectively. The primer extension analysis was carried out and the initiate sites of promoter cassette were determined.