链霉菌碱性脂肪酶的研究

STUDIES ON ALKALINE LIPASE BY STREPTOMYCES

从杭州土壤中获得的一支链霉菌，通过ＵＶ、ＤＥＳ和Ｃｏ６０ － γ射线的５ 代诱变，选育成功了高产的Ｚ９４－ ２ 菌株，经过对Ｚ９４ －２ 的发酵研究，产碱性脂肪酶活力达５９６ ｕｍＬ，其最适培养基（λｕ Ｌ－１） 为：糊精１０、黄豆饼粉３０、尿素１０ 、Ｋ２ＨＰＯ４０ ．５、ＭｇＳＯ４·７Ｈ２Ｏ０ ．５、ＮａＣｌ１ 和ＡＥＯ９ ０．５ ，产酶的最适条件为：初始ｐＨ９．０～１０．０ ，２６℃培养４８ ｈ．对Ｚ９４－ ２

Streptomyces Z94-2, a alkaline lipase producing strain, was obtained through mutation for 5 generations by UV, DES and Co 60 - γ from a wild strain in the soil in Hangzhou. The optimal medium for the strain ( ρ /g L -1 ): soybean meal 30, urea 10, dextrin 10, KH 2PO 4 0.5, MgSO 4·7H 2O 0.5, NaCl 1 and AEO 9 (nonionic surfactant) 1, and the optimal conditions for lipase production: initial pH 9.0～10.0, culture temperature 26℃ and culture time 48 h. The maximal lipase activity was 596 (u/mL).The enzyme characteristics of Streptomyces Z94-2 were compared with those of other strains.