RP-HPLC法同时测定白头翁汤中盐酸小檗碱、盐酸药根碱、盐酸巴马汀的含量

Simultaneous determination on the contents of berberine hydrochloride,jatrorrhizine hydrochloride and palmatine hydrochloride in Pulsatill Decoction by RP-HPLC

目的:建立反向高效液相色谱方法同时测定白头翁汤中盐酸小檗碱、盐酸药根碱、盐酸巴马汀的含量。方法:色谱柱为XP ODS-A C18(250 mm×4.6 mm,5μm);采用线性梯度洗脱方法,流动相:溶液A为乙腈,溶液B为0.05 mol/L磷酸二氢钾(磷酸调pH值至3.0)。0-40min溶液A∶溶液B为5∶95-45∶55;流速:1.0 mL/min;柱温:室温;检测器:DAD检测器;检测波长:340 nm;进样量1:0μL。结果:盐酸小檗碱、盐酸药根碱和盐酸巴马汀分别在62.5-1 000μg/mL、46.875-750μg/mL和37.5-600μg/mL范围内线性良好;平均加样回收率分别为99.22%(RSD为3.25%)、98.46%(RSD为1.99%)和99.20%(RSD为1.69%)。结论:本方法简便、灵敏、准确,适用于白头翁汤的质量控制。

Objective： To establish a simultaneously determination on the contents of berberine hydrochloride, jatrorrhizine hydrochloride and palmatine hydrochloride in Pulsatill Decoction by RP-HPLC. Methods： With the method of linear gradient elution, the chromatographic column was XP ODS-A C18 （ 250 mm×4.6 ram, 5 μm）, the mobile phase was stated as follows： solution A was acetonitrile, and solution B was 0.05 mol/L KH2PO4 （adjust pH = 3.0 with H3PO4）. The ratios of A/B a! 0-40min was 5：95-45：55. The flow rate was 1.0mL/min, column temperature was 25℃ , DAD detection wavelength was 340 nm. and injection vohmm was 10μL. Resuhs： The good linear relationship of berberine hydrochloride, jatrorrhizine hydrochloride and palmatine hydrochloride were obtained in the range of 62.5-1000 μg/mL, 46.875-750 μg/mL and 37.5-600 μg/mL, respectively. The average recovery was 99.22%（ RSD=3.25% ）, 98.46%（RSD= 1.99%） and 99.20 %（RSD= !.69% ）, respectively. Conclusion： This method was convenient, sensitive and aceurate. It could be used to control the quality of Pulsatill Decoction.