分子印迹电化学传感器测定L-色氨酸的研究

A Molecular Imprinting Electrochemical Sensor for L-Tryptophan Determination

报道了一种由溶胶-凝胶法制备的分子印迹电化学传感器,并用于L-色氨酸的测定。印迹聚合物是由四乙氧基硅烷、甲基三甲氧基硅烷、苯基三甲氧基硅烷等聚合而成,L-色氨酸为模板分子。通过循环伏安法验证了印迹膜与模板分子的结合和去除。该传感器对L-色氨酸具有良好的选择性以及高的灵敏度,线性范围为1.0×10-9～1.0×10-5 mol/L(r=0.9958),检出限为1.0×10-12 mol/L。该法可不经分离直接用于人血清中L-色氨酸的测定,回收率在80.0%～104%范围。

A molecular imprinting electrochemical sensor for the determination of L-tryptophan was developed.The molecular imprinting polymer（MIP） was fabricated by the polymerization reaction of ethyl silicate（TEOS）,phenyltrimethoxysilane（PTMOS） and phenyltrimethoxysilane（MTMOS）.The presence and removal of the template molecule of L-tryptophan in the MIP film were confirmed by cyclic voltammetry.The sensor showed an excellent selectivity and a high sensitivity for L-tryptophan detection,with a linear range from 1.0×10-9 to 1.0×10-5 mol/L（r=0.9958） and a limit of detection as low as 1.0×10-12 mol/L.The sensor was successfully applied to the determination L-tryptophan in the serum samples and the recoveries were in the range of 80.0%~104%.