细胞色素c在纳米杂化膜修饰玻碳电极上的直接电化学

Direct Electrochemistry of Cytochrome c at Nanohybrid Film Modified Glassy Carbon Electrode

以多壁碳纳米管(MWNTs)修饰玻碳(GC)电极为基底,自组装金纳米粒子(AuNPs)及L-半胱氨酸(L-Cys)研制杂化膜修饰电极(L-Cys/AuNPs/MWNTs/GC)。实验表明,该膜修饰电极在pH=7.0的KH2PO4-K2HPO4缓冲溶液中对细胞色素c(Cyt c)的直接电子转移反应具有良好的电催化作用,Cyt c于0.121V(vs.Ag/AgCl)附近产生一对可逆的氧化还原峰,峰电流与其浓度在4.0×10-5～3.2×10-4 mol/L范围内呈现良好的线性关系,线性相关系数为0.9957,检出限为2.4×10-5 mol/L。该传感器响应快速,性能优良。

L-cysteine（L-Cys）,gold nanoparticles（AuNPs） and multi-walled carbon nanotubes（MWNTs） were immobilized on glassy carbon（GC） electrode surface by layer-by-layer self-assembly technique to form a robust and effective nanohybrid film.The modified electrode（L-Cys/AuNPs/MWNTs/GC） can promote the direct electron transfer reaction of cytochrome c（Cyt c）.A pair of well-defined and nearly reversible peaks of Cyt c at about 0.121 V（vs.Ag/AgCl） was obtained.The sensor responded rapidly to Cyt c in the linear range from 4.0×10-5 to 3.2×10-4 mol/L with detection limit of 2.4×10-5 mol/L in PBS（pH=7.0）.